In this work, we modified a hydrophobic electrospun poly (L-lactic) acid (PLLA) film with poly (sulfobetaine methacrylate) (pSBMA)-catechol conjugates of different molecular weights to improve the biocompatibility of the film. These conjugates were synthesized via atom transfer radical polymerization. They consist of an ultra-low fouling pSBMA zwitterionic polymer with a surface-adhesive catechol moiety. X-ray photoelectron spectroscopy, contact angle and scanning electron microscopy experiments were performed to characterize films before and after modification with pSBMA-catechol conjugates. Enzyme-linked immunosorbent and fluorescently-labeled bovine serum albumin were used to study the interactions of proteins with these films. Results showed that low molecular weight zwitterionic pSBMA-catechol conjugates greatly discouraged protein adsorption as shown by use of single protein solutions on PLLA films when the modification was performed in ethanolic Tris-HCl solution. This work offers a convenient and effective method to modify electrospun PLLA films for biomedical applications. Statement of Significance In this work, we report a convenient and effective method to modify electrospun PLLA films using pSBMA-catechol conjugates via "graft-to" for biomedical applications. After pSBMA modification, the PLLA surface becomes hydrophilic with low contact angle and protein adsorption. Results showed that lower molecular weight zwitterionic pSBMA-catechol conjugate led to lower contact angles and better nonfouling properties on PLLA films when the coating was performed in a solution containing ethanol. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.