The aim of this study was to develop erythrosine-loaded electrospun polyvinyl pyrrolidone/hydroxypropyl-beta-cyclodextrin (PVP/HP beta CD) fiber patches for plaque disclosing. The fiber patches had been fabricated via electrospinning process and were characterized using scanning electron microscope (SEM), Fourier transform infrared spectrophotometry (FT-IR), differential scanning calorimeter (DSC) and powder X-ray diffractometry (PXRD). The fiber patches were then further evaluated for wetting and integration abilities, mechanical properties, loading efficiency, loading capacity and release characteristic. These erythrosine-loaded fiber patches exhibited excellent wetting and disintegration properties and prompt released the erythrosine form the patches after contacted with saliva. These erythrosine-loaded fiber patches could be exploited as a plaque-disclosing material. However, further in vivo studies are needed for the application.