This study investigates the effect on: (1) the bulk surface and (2) the three-dimensional non-woven microfabric scaffolds of poly(N-isopropylacrylamide)CNTpolyaniline on growth and viability of cells. The poly(N-isopropylacrylamide)CNTpolyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, non-woven microfabric scaffolds. The electrospun microfabrics were assembled in regular three-dimensional scaffolds with OD: 400500 m; L: 620 cm. Mice fibroblast cells L929 were seeded on the both poly(N-isopropylacrylamide)CNTpolyaniline bulk surface as well as non-woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N-isopropylacrylamide)CNTpolyaniline non-woven microfabric matrices in compare to poly(N-isopropylacrylamide)CNTpolyaniline bulk and commercially available Matrigel even with a range of cell lines up to 168 h. Temperature dependent cells detachment behavior was observed on the poly(N-isopropylacrylamide)CNTpolyaniline scaffolds by varying incubation at below lower critical solution temperature of poly(N-isopropylacrylamide). The results suggest that poly(N-isopropylacrylamide)CNTpolyaniline non-woven microfabrics could be used as a smart matrices for applications in tissue engineering. (c) 2012 Wiley Periodicals, Inc. Biopolymers 99: 334341, 2013.