Cellulose-based materials are one of the most commonly used materials for biomedical applications, which normally applied as carriers for pharmaceuticals and drug-releasing scaffolds. In this study, cellulose acetate (CA) was used to fabricate the nanofibrous membrane using the electrospinning technique. CA solutions at different concentrations were prepared by dissolving the polymer in a mixture of acetic acid/acetone solvents with the ratio of 3 : 1. The field emission scanning electron microscope results showed that electrospinning of 10% (w/v) CA produced nanofibres with many beads. When the CA concentration was increased to 14% (w/v), bead-free nanofibres were produced. The contact angle measurement results confirmed the hydrophilic properties of nanofibres. In order to prevent common bacterial infections, a model drug, Tetracycline a <... HCL was incorporated into the CA nanofibres. The drug-loaded CA nanofibres showed antibacterial activity against Gram-positive and Gram-negative bacteria. CA nanofibres had high water uptake properties. The CA nanofibrous membrane was non-toxic to human skin fibroblast cells. Thus the CA nanofibres with 14% (w/v) concentration exerted suitable properties for wound healing application.