The use of fine fiber has become an important design tool for filter media. Nanofibers-based filter media have some advantages such as lower energy consumption, longer filter life, high filtration capacity, easier maintenance, low weight rather than other filter media. The nanofibers-based filter media made up of fibers of diameter ranging from 100 to 1,000 nm can be conveniently produce by electrospinning technique. Common filter media have been prepared with a layer of fine fiber on typically forming the upstream or intake side of the media structure. The fine fiber increases the efficiency of filtration by trapping small particles, which increases the overall particulate filtration efficiency of the structure. Improved fine fiber structures have been developed in this study in which a controlled amount of fine fiber is placed on both sides of the media to result in an improvement in filter efficiency and a substantial improvement in lifetime. In the first part of this study, the production of electrospun nanofibers is investigated. In the second part, a different case study is presented to show how they can be laminated for application as filter media. Response surface methodology (RSM) was used to obtain a quantitative relationship between selected electrospinning parameters and average fiber diameter and its distribution.