A theoretical model for the morphology transition of short and continuous nanofibers by electrospinning has been proposed. The influences of polymer concentration, applied voltage, and flow rate on the fabrication of short and continuous nanofibers were mapped for use as a reference in the design and construction of the theoretical model. The morphology transition of short and continuous nanofibers occurred mainly due to changes in the flow rate and voltage. According to the concentration of the polymer in the solution, the map of the short nanofiber region was narrowed as the polymer concentration increased. The theoretical model derived from the conservation of kinetic energy and potential energy experienced by the polymer solution resulted in an equation that could be used to calculate the voltage and flow rates under certain boundary conditions when cutting nanofibers. The boundary conditions for voltage were 4.7-4.9 kV, and the boundary conditions for flow rate were 0.1-1.1 mu l/min.