The uncrosslinked wound dressing with core. shell nanofibers was firstly fabricated via co-axial electrospinning. The nanofiber was composed of the polycaprolactone(PCL) core loaded with anti-bacterium drug mupirocin and collagen shell with anesthesia lidocains. The surface morphology of uncrosslinked dressing and its core. shell structured fibers were characterized by scanning electronic microscopy and transmission electronic microscopy, respectively. The crosslinked wound dressing can be further prepared by the crosslinking of collagen shell, and the successful crosslinking can be proved by the enhanced tensile strength and swelling ratio. In vitro drug release tests implied two dressings both possessed the burst release stage and sustainable release stage for two loaded drugs, which confirmed the sustainable acesodyne and anti. bacteria effects for both dressings. The bicinchonininc acid(BCA) test results showed that the collagen in uncrosslinked dressing could also be sustainably released, while in vitro cell attachment test depicted that uncrosslinked dressing could promote the cell attachment and growth better than crosslinked dressing. Furthermore, the in vitro anti. bacteria test showed that both dressings loaded with mupirocin had significantly better anti-bacteria ability than those without mupirocin indicating their excellent anti. bacteria ability. Two as-prepared multifunctional wound dressings possessed acesodyne and anti. bacteria effects and were able to promote the healing process so that they can reduce the pains of patients, reduce the number of dressing change, and enhance treatment effect.