Nanotechnology has wide applications in many fields, especially in the biological sciences and medicine. Nanomaterials are applied as potential materials for treatment and diagnosis. The development of nanofibers has greatly enhanced the scope for fabricating designs that can be potentially used in medical sciences. The application of polymeric nanofibers in biomaterials sciences and tissue engineering review in four sections: skin and eye, neural and cardiovascular tissues, musculoskeletal and urological tissues, drug and biological materials' delivery. The present review summarizes the currently available applications of nanofibers in skin and eye tissues.