We systematically studied the structure, magnetism, resistance, magnetoresistance (MR) and nonlinear current-voltage (I-V) curves of La0.33Pr0.34Ca0.33MnO3 (LPCMO) nanofibers via electrospinning and followed calcination. Magnetic study shows that this nanosystem can form long-range order and generate spin-glass states at low temperatures. The resistance and MR studies indicate the presence of metal insulator transitions and colossal MR effects in the system, respectively. One-dimensional variable range hopping (1D VRH) model is used to explain the high-temperature segment behavior of resistance and MR. The behavior of the nonlinear I-V curves indicates the exist of threshold voltage and zero bias conductance at low temperatures in the LPCMO nanowires, which represents the existence of Coulomb gap.