Carbohydrates derivatives containing propargyl pending functional groups have been used in medical applications due to their improved chemical, biological, and functional properties. In this work, azide and alkyne pendant functional groups were introduced onto ethyl cellulose (EC) and beta-cyclodextrin respectively in order to demonstrate the ability of scaffold formation by click chemistry. Technically, Azido-ethyl cellulose (D.S.(Azido) 0.19) and 2, 3, 6-O-propargylated beta-cyclodextrin were synthesized and characterized as click-triggered candidates. The click-gel scaffold was obtained by mixing the two novel candidates in the presence of copper catalyst. Azido-EC was considered to produce clickable electrospun fibers blended with EC (290-620 nm) at 20 kV in order to functionalized EC substrate bearing azide groups to be amenable towards immobilization of an alkyne-terminated bio-molecules. The biocompatibility assay of the prepared click-triggered candidates showed very high level of viability in the human skin fibroblast cells (HFB4) at concentration 100 mu g/ml.