Polymer nanofibers have a specific set of material properties that are favorable for many applications in biomedical engineering (scaffolds, stents or tissues engineering). In this article, influence of the electrostatic field distribution on the fiber deposition pattern is examined. The electrode pair used in the study is part of a lineal quadrupole trap used in ion trapping experiments. The impact of the bias potential on the nanofiber collection patterns and the electrospinning process is examined through a series of experiments and finite-element analysis of the resulting field distribution. A set of optimal amplitudes of the steering electric field components is also reported.