Electrospun blended polymeric nanofibers have been prepared with fabrication of hydroxyapatite (HAp) nanoparticles for improve the effect of cartilage regeneration applications. The nanofibrous structure, morphology and chemical interactions were successfully investigated by efficient analytical methods. The crosslinking of blended polymer with HAp particles was confirmed by FTIR and XRD spectroscopic techniques. The incorporation of nano-scaled HAp particles into the structure of blended nanofibers, frequent spherical particles appeared within the nanofibers which demonstrate the successful amalgamation of HAp nanoparticles within the PCL/PGS nanofibrous membrane. The diameter of blended polymeric nanofibers was increased with addition of HAp nanoparticles, which was confirmed by FE-SEM images. The cell responses (adhesion morphology, proliferation and viability) of prepared nanofibrous scaffold were examined on chondrocyte type bone marrow-derived mesenchymal stem cells (BMSCs) in vitro. These results confirmed that HAp incorporated blended nanofibrous scaffolds exhibited excellent biocompatibility and osteoblast adhesion properties. Therefore, the facile structurally improved nanofibrous scaffolds could be used in the articular cartilage regeneration.