Electrospun nanofibers are researched for protective applications. Ag nanoparticles promote antibacterial properties and MgO enhances fire retardancy. The main objective of this research was to develop Ag/MgO/Nylon 6 electrospun nanofibers that are scarcely reported. Nanofibers were synthesized using formic acid and acetic acid solvents and collected on cotton fabric. Nylon 6 in 20 wt% along with MgO in 3-5 wt% and AgNO3 in 0.25-0.75 wt% was used for electrospinning. Nanofibers of diameter 35-55 nm with no beads were obtained for MgO (5%)-AgNO3 (0.5%)/Nylon 6. Bacterial reduction of 88% for MgO (3%)-AgNO3(0.25%)/Nylon 6 against Staphylococcus aureus bacteria and 54% against Escherichia coli was achieved. Nanofibers of MgO (3%)-AgNO3 (0.25%)/Nylon 6 and MgO (4%)-AgNO3 (0.5%)/Nylon 6 were rated V-0 in vertical burning test. Least burning rate of 1.56 mm/s corresponded to nanofibers of MgO (3%)-AgNO3 (0.25%)/Nylon 6 in horizontal burning test. Energy Dispersive X ray (EDX) confirmed the presence of Mg, O, and Ag. Fourier Transfer Infrared Spectroscopy (FTIR), the stretching of O-H and CH2.