Silver nanoparticles (AgNPs), reduced from AgNO3 in a nylon-6 solution, were immobilized through electro-spinning on polypropylene (PP) to form an AgNP-nylon-6/PP package film. The AgNP-nylon-6/PP films UV/VIS spectra exhibited absorption bands at 410 and 425 nm, which were typical surface plasmon polariton resonances of the two AgNP forms. The 2-5 nm AgNPs, which were face-centered cubic crystal with (111) plane-type structures contributing to antimicrobial ability, were uniformly distributed on the nanofibers. The inhibition percentage of AgNP-nylon-6/PP composite against Escherichia coli and Staphylocccus aureus could reach 100% when the accumulative release amount of Ag+ was less than 0.62 mu g/mL. The release ratio, k and n of the release kinetics evaluated by Fickian diffusion of Ag+ in food simulants were in the order of 3% acetic acid > RO water > 10% alcohol > 95% alcohol.