Bimetallic Au-Ag/Co3O4 nanofibers (Au-Ag/Co(3)O(4)NFs) were synthesized by an electrospinning method and calcination treatment. Due to the admirable synergic effect of electrocatalytic ability of Au and Ag, and the large surface-to-volume ratio of Co3O4 nanofibers caused by their rough surface, the synthesized Au-Ag/Co(3)O(4)NFs were applied to a sensing platform for hydrogen peroxide oxidation. Using electrochemical techniques, the novel sensing platform based on Au-Ag/Co(3)O(4)NFs shows a wide linear concentration range with high detection sensitivity of 1241.1 mu A mM(-1) cm(-2). This sensitive sensor was also applied to determine H2O2 from human breast cancer cells, indicating its great potential application in physiology and pathology. (C) 2018 Elsevier B.V. All rights reserved.