To investigate the potential of nanoweb laminates that contain juniperus chinensis extracts as functional garments or specialty textiles, a layered fabric system with PU nanoweb was inserted between the PET fabric and the mesh. Of particular interest in this study was the development of nanoweb laminates by electrospraying polyurethane resin onto the substrate fabrics. Therefore, to minimize damage to the nanoweb, the PU adhesive resin was electrosprayed on the polyester plain weave fabric and mesh, respectively, and the PU nanoweb laminate was prepared by inserting PU nanowebs containing juniperus chinensis extracts (JCE) between two fabrics and heat-pressing them together at 110 degrees C for 30 s. To examine the compatibility of the fabricated PU nanoweb laminates (PNLs), 1, 5, and 10 launderings were performed, and changes in the physical and functional properties due to the laundering were investigated. The nanofiber structure of the PNL was maintained after repeated laundering. The tensile strength of the PNL was maintained partially after repeated laundering. There was no significant change in the moisture permeability after repeated laundering, whereas the air permeability decreased somewhat after the launderings, but an acceptable level of air permeability still was maintained. Laundering made the PNLs less waterproof, and their adhesive strength was reduced, which implied that different laminating techniques or substrate materials should be investigated. However, the anti-bacterial properties of the PNLs were very good for both Staphylococcus aureus and Pneumoniae.