Electrospun chitosan-polyethylene oxide nanofiber mats were fabricated using a new collector design. Besides being reusable, it allows to assess the desired morphology of the mat in a quicker way. To test its efficiency, nonwoven mats designed for water treatment applications were prepared using conditions never been reported before. Under these conditions, continuous and flawless nanofibers of 151 +/- 36 nm in diameter were achieved. Adsorption capacity of the mats for copper ions in aqueous solutions were investigated. Results showed that sorption equilibrium was achieved within 150 min with a homogenous distribution of copper ions within the nanofibrous mats. The pseudo-second order kinetic model best fitted the experimental data. The Langmuir isotherm best described the sorption process with a maximum adsorption capacity of 124 mg/g for trial temperatures ranging from 25 to 60 degrees C. Thermodynamic parameters (Delta G degrees, Delta H degrees and Delta S degrees) demonstrate that the adsorption was feasible, endothermic and spontaneous. The desorption potential and mat's reusability were also studied. Results reveal that the electrospun chitosan mats can be desorbed and reused up to 5 cycles without significant loss in adsorption performance.