In the present work, the biodegradable nanofibrous scaffolds containing poly(caprolactone) (PCL), poly(vinyl alcohol) (PVA) and aloe vera (AV), through two nozzles electrospinning method, were prepared and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement and mechanical analysis. The biocompatibility and cell growth were evaluated using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Adding poly(vinyl alcohol) (PVA) and poly(caprolactone) (PCL) boosted the electrospinability of aloe vera (AV) solution and mechanical features of the scaffolds. According to the Scanning electron microscopy (SEM) results, uniform PVA/AV-PCL nanofibers were fabricated with the average diameter about 119 +/- 11.78 nm. Fourier-transform infrared spectroscopy) FTIR (confirmed the presence of functional groups of scaffolds. The results of the contact angle showed that by adding aloe vera, the hydrophilicity increased. The results showed that the tensile strength of samples with 6 mg/mlAV, reached to 2.02 MPa. MTT results showed that all samples had a cell viability of over 80 %. It can be concluded that nanofibrous scaffolds with concentration of 6 mg/ml, distance between syringe tip/collector of 15 cm, feed rate of 3 ml/h and applied voltage of 12 kV, have appropriate properties for skin tissue engineering by stimulating the fibroblast cells and therefore accelerating the regeneration processes of damaged skin.