In this report, the inclusion complexes of eugenol-gamma cyclodextrin (gamma CD) were incorporated in pullulan nanofibers using electmspinning technique. As a control sample, the pristine eugenol contained pullulan nanofibers were electrospun, as well. The nanofibmus webs were prepared with the initial eugenol content of 12% (w/w) and pullulan/eugenol-gamma CD nanofibers could preserve similar to 93% of this volatile essential oil compound due to inclusion complexation. On the other hand, only similar to 23% of eugenol was preserved in case of pullulan/ eugenol nanofibers. The inclusion complexation also provided an enhanced thermal stability for eugenol and its volatilization shifted from similar to 50-190 degrees C to similar to 125-300 degrees C in case of pullulan/eugenol-gamma CD nanofibers. The antioxidant performance of eugenol was not affected by the inclusion complexation and the nanofibers of pullulan/ eugenol-yCD and pullulan/eugenol indicated similar to 100% and similar to 58% radical scavenging activity for the same nanofiber concentration (250 mu m/mL). Moreover, pullulan/eugenol-gamma CD nanofibers maintained the antioxidant activity even after 3-months storage at room temperature (similar to 98%) and heat-treatment performed at 175 degrees C for 1 h (similar to 93%). The time dependent release test indicated that pullulan/eugenol-gamma CD nanofibers has a release profile in a relatively controlled manner compared to pullulan/eugenol nanofibers under the same experimental conditions. Here, the functional nanofibrous web encapsulated an essential oil compound was developed using the edible pullulan and non-toxic and natural cyclodextrin molecules. Therefore, pullulan/eugenol-gamma CD nanofibers can be quite a promising encapsulation and carrying matrix for the essential oils which are widely used in both food and pharmaceutical areas.